Metallo- beta-lactamases among multidrug resistant [MDR] gram negative bacteria isolated from clinical specimens during 2009 in Sanandaj, Kurdistan province

ABSTRACT

Today, there are numerous reports about emerging multi drug resistant gram negative bacteria all around the world, especially in ICUs. Rarely, Metallo-beta-lactamase [MBL] enzymes are responsible for these cases. Study of MBLs for diagnosing and preventing distribution of the origin of infection are critical issues. In addition, we would like to compare the efficacy of Iranian and foreign- made antibiotic disks. During 2009 all entered clinical specimens to the laboratory tested for detecting gram negative bacteria. Isolated bacteria were tested by Kirby-Bauer method to antibiotic susceptibility test by Iranian and foreign [MAST] disks. For gram negative carbapenem resistant isolates, PCR technique used to detect VIM, GIM, and SIM variants of MBLs. During one year, 17890 clinical specimens referred Besat laboratory. The most specimen was Urine [8172] followed by blood culture [5190] that in which 1110 gram negative and positives isolated. Out of which, 778 [70%] of isolates were gram negatives. MDR gram negatives were 157 [20.2%]. Imipenem and meropenem were the most efficient antibiotics [all susceptible] and ceftriaxone was the least [19% susceptible]. E. coli was the most prevalent isolate. 79 Gram negative isolates [10.1%] were resistant to Iranian-made discs but all susceptible for foreign ones. All 79 isolates were tested by PCR for MBL genes, that, all were negative. Besides, Iranian imipenem and cefepime disks have had distinguishable difference in susceptibility of isolates. Fortunately, none of gram negative isolates were MBL producer, which revealed no colonization of MBL producing bacteria. Iranian-made disks appear efficient except for imipenem and cefepime.