Polymerase chain reaction techniques: applications in parasitic diseases

ABSTRACT

dNTPs Nucleotides; ESTs Expressed sequence tags; HAT-RAPD-PCR High annealing temperature random amplified polymorphic DNA PCR; HSP Heat shock protein; ITS Internal transcribed spacer; mRNA Messenger RNA; MSP Merozoite suiface proteins; MT-PCR Multiplex tandam PCR; PCR Polymerase chain reaction; qPCR Quantitative basic PCR; qRtime-PCR Quantitative real-time PCR; qRT-PCR Quantitative reverse transcriptase PCR; QT-NASBA Quantitative nucleic acid sequence-based amplification; RAPD Random amplified polymorphic DNA; RFLP Restriction fragment length polymorphism; RNA Ribonucleic acid; Rtime-PCR Real time PCR; RT-PCR Reverse transcriptase PCR; SNP Single nucleotide polymorphism;TLRs Toll-like receptors