Spectrophotometric analysis of cefepime through its Hg [I] complex

ABSTRACT

A spectrophotometric procedure -was developed for determination of cefepime depending on the complexation of the drug with Hg[2][NO[3]]2 in acid medium [pH 3.5] and measuring the absorbance at 263 nm. Different variables affecting the reaction were studied and optimized. Under the optimized conditions, linear relationship with good correlation coefficient [0.999] was found between the absorbance and the concentration in the range of 3.65-40 microg ml[1] . The limits of detection and quantitation were 1.20 and 3.65 microg mt[1] respectively. The stoichiometry of the reaction was studied using Yoe and Jones method and was found to be 12 ratio for cefepime Hg [I]. The method was successfully applied for determination of cefepime in its vial with average percentage recovery of 98.95 +/- 1.079. The results were favorably compared with those of reference method. The IR study of the formed complex was done and different probabilities of the formed complexes were suggested