Detection and characterization of verotoxin-producing strains among sorbitol non-fermenting Escherichia coli
EMHJ-Eastern Mediterranean Health Journal. 2001; 7 (4-5): 756-762
en Inglés
| IMEMR
| ID: emr-157992
ABSTRACT
The presence of genes for verotoxin 1 and 2 [VT1 and 2] among sorbitol non-fermenting Escherichia coli isolates from diarrhoeal cases was assessed using polymerase chain reaction assay. Of 60 [88%] positive isolates, 37 [62%] harboured VT1 and 23 [38%] both VT1 and VT2. In HeLa cell adherence assay, 48 [71%] isolates exhibited mannose-resistant adherence to HeLa cells. Multidrug resistance was observed in 56 [82%] isolates, with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim and tetracycline pattern being the most common. There were 13 common and 22 single biochemical phenotypes identified. Isolates belonging to common biochemical phenotypes normally had a similar pattern of adherence and VT production, but differed greatly in their pattern of antibiotic resistance, pointing to a high rate of antibiotic-resistance transfer among these isolates
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Sorbitol
/
Adhesión Bacteriana
/
ADN Bacteriano
/
Dermatoglifia del ADN
/
Técnicas de Cultivo de Célula
/
Escherichia coli O157
/
Toxina Shiga I
/
Toxina Shiga II
/
Farmacorresistencia Bacteriana Múltiple
/
Diarrea
Límite:
Niño
/
Humanos
Idioma:
Inglés
Revista:
East Mediterr Health J.
Año:
2001
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