Evaluation of the COBAS AMPLICOR MTB test for the detection of Mycobacterium tuberculosis complex
EMHJ-Eastern Mediterranean Health Journal. 2004; 10 (3): 329-335
en Inglés
| IMEMR
| ID: emr-158291
ABSTRACT
We evaluated the COBAS AMPLICOR polymerase chain reaction [PCR] based test for the detection of Mycobacterium tuberculosis complex in 866 respiratory and non-respiratory samples. Acid-fast staining and culture on Lowenstein-Jensen medium were also performed on all samples. Of the 866 samples tested, 87 [10.0%] were PCR-positive compared to 94 [10.9%] culture positive. There were no false positive results but 7 PCR-negative, culture-positive samples were, considered false negatives after reviewing medical records of patients. A PCR inhibitory rate of 2.0% [17/866] was observed in respiratory samples only. Sensitivity, specificity, and positive and negative predictive values for this test were 92.5%, 100%, 100% and 99.1% respectively. This test is a valuable diagnostic tool for today's mycobacteriology laboratory
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Tuberculosis
/
Biopsia
/
Líquido del Lavado Bronquioalveolar
/
Líquido Cefalorraquídeo
/
Sensibilidad y Especificidad
/
Técnicas de Cultivo de Célula
/
Colorantes
/
Reacciones Falso Positivas
Límite:
Humanos
Idioma:
Inglés
Revista:
East Mediterr Health J.
Año:
2004
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