Association between the polymorphisms of angiotensin converting enzyme [Peptidyl-Dipeptidase A] INDEL mutation [I/D] and Angiotensin II type I receptor [A1166C] and breast cancer among post menopausal Egyptian females

ABSTRACT

Renin-angiotensin system [RAS] is involved in the regulation of cardioascular homeostasis. Angiotesin [Ang II] is converted from angiotensin converting enzyme [ACE]. Ang II exerts it effects by binding to two types of receptors; AT[1]R and AT[2]R. Ang II effect on AT[1]R promotes proliferation angiogenesis and metastasis in breast tissues. ACE [I/D] polymorphism is an insertion/deletion of a 287 bp DNA fragment within intron 16 of ACE gene. A1166C is a single nucleotide polymorphism [SNP] in the 3-UTR of AT[1]R gene. Both [D] and [C] alleles were found to be related to RAS overactivation. One hundred and twenty postmenopausal Egyptian females were included in the present study and were divided into control group [fifty apparently healthy women] and patients group [seventy breast cancer patients]. Detailed history taking was done with stress on age, family history, menstrual, obstetric, medical and drug history. Physical examination including body mass index calculation was done. Histopathological examination was done for tumor grading and staging. Detection of ACE gene [I/D] polymorphism by PCR and AT[1]R AT[1]66C SNP using PCR/RFLP were done