Protein content study revealed presence of isoform 2 of beta-tropomyosin in primary breast cancer tissues from Sudanese patients

This study was designed to compare antigen content of normal and cancerous breast tissues of Sudanese patients. 50 tissue samples- normal and cancerous - from 25 Sudanese patients with primary breast cancer were analyzed for their protein content using 2D PAGE and for protein identification using LC/MS and nr. fasta data base search. Beta-Tropomyosin spot was found in all the cancerous tissues and absent from all the normal tissues of the same patients. The protein is isoform 2 with 257 amino acids. Primary breast cancer tissues from Sudanese patients are characterized by the presence of isoform 2 of beta-tropomyosin, which is not detected in the normal tissues