[Determination of peroxisome proliferator-activated receptorgamma polymorphism in patients with helicobacter pylori infection]

ABSTRACT

Peroxisome proliferator-activated receptor gamma [PPARgamma] is a ligand-dependent transcription factor involved in various disease processes including inflammation and carcinogenesis .This study aimed to determine polymorphism of PPARgamma gene and its association with Helicobacter pylori infection and gastrointestinal diseases in patients. Two hundred patients with helicobacter pylori infection were examined. H. pylori infection was diagnosed by histology, rapid urease test [RUT], culture, ELISA and PCR. PPARgamma polymorphism was analyzed by PCR-based restriction fragment length polymorphism. In total 200 patients [4 gastric cancer, 141gastritis, 35 peptic ulcer, 18 duodenal ulcer, 2 peptic ulcer and duodenal ulcer] with Helicobacter pylori infection were enrolled .The frequency of PPARgamma G [Ala12] allele [5%] showed a significant association with gastric cancer [P=0.004] or gastritis [P=0.007]. PPARgamma GC [Pro12Ala] allele [35%] showed a significant association with duodenal ulcer [P=0.03] or gastritis [P=0.002]. Pro12Ala PPARgamma polymorphism is associated with gastric cancer and gastritis and is a potential marker for genetic susceptibility to these two diseases in the presence of H. pylori infection. Finally, our study suggests the potential association between PPARgamma polymorphism and H. pylori infection in the development of gastric cancer and peptic ulcer and gastritis