Protein pattern of penicillium species isolated from natural sources in Iran

ABSTRACT

Farctionation of diffecent penicillium species based on protein bands. In this study penicillium citrinum, penicillium oxalicum, penicillium notatum and penicillium frequentes isolated from air in Iran have been compared for their protein pattern antigens. First, the isolates were cultured on sabouraud dextrose agar medium and then subcultured on czapex agar and were maintained on 30°C for 48-72h. Then they were cultured on sobouraud broth medium for preparing protein extracts, and braudford method was used for measuring the level of protein. The proteins were differentiated using SDS-PAGE with 10% separating gel. Coomassie blue G250 was used for staining. 34 protein band with molecular weight of 19.5, 24, 26, 27, 28.5, 32, 36, 39, 45, 48, 50, 52, 53, 55, 56.5, 59.5, 63, 65, 66.5, 68, 76, 84, 88, 90, 92, 93, 94, 95, 97, 107, 116, 123, 128 and 158 kD were observed. The bands 19.5, 24, 28.5, 45, 52, 53, 56.5, 59.5, 76, 84 and 97kD were present in all 30 isolates under study. The results, indicate that there are inter species and intra species differences but there is no significant difference in protein patterns of the isolates