effects of baclofen [GABA-B receptor agonist] administration alone and accompanied with LRH-A and metoclopramid on GTH-I release in carp [Cyprinus caipio L.]

ABSTRACT

Investigation of possibility and the role of aminobutyric acid [GABA]in the fish pituitary functional regulation ,by measuring the changes in blood serum GTH-I levels. Experimental study. Fifty sexually regressing female carps. Seven experimental conditions were tested.Injection with just normal saline [control or basal] and intraperitoneal injection of Bac.[9 mg/kg], LRH-A [50 microg/kg], Met.[15 mg/kg], Bac.+LRH-A, Met.+LRH-A, Bac.+ Met.+LRH-A were done. In this study fishes were bled imediately before injection and 5 hours after then. Statistical analysis: Pre and post-treatment GTH-I values were compared by t-test and Mann-Whithney U test. Experiments indicated that only the injection of baclofen alone could produce a significant decrease in the serum GTH-I levels. Other factors, whether alone or in combination with each other did not produce acceptable changes in GTH-I secretion at least in this stage of sexual cycle. The results of the present study suggest that GAB A may be one modulator for serum GTH-I levels in the sexually regressing female carps