Analysis of protection effect of vitamins A, E against Gamma induced chromosomal abnormalities post and past-irradiation on mouse bone marrow cells usingmicronucleus assay

ABSTRACT

The radioprotectory effects of vitamins A and E post and past Gamma-irraiation on mouse bone marrow cells using micronucleus assay was analyzed. Micronucleus assay is a very fast and effective method in evaluating, structural as well as numerical chromosomal abnormalities. Search for find/ng an effective way of protection against harmful effects of induced or spontaneous irradiation is actively progressing. Effects of antioxidants such as vitamin A and E in in vivo have studied extensively. In this study effect of different doses of vitamin A and E post and past Gamma-irradiation of 2 Gy wererepresenting chromosomal damages. Of all doses used [0.6mg/kg, 1.2mg/kg and 2.4mg/kg for vitamin A and 50mg/kg, l00mg/kg and 200mg/kg for vitamin E/ l.Amg/kg, body weight of vitamin A and 200mg/kg body weight of vitamin E had the best effect on reducing induced-damages. Combination of two vitamins in these doses post and past irradiation revealed that vitamin E was the most effective post irradiation whereas vitamin A was the most effective past irradiation in reducing the frequency of micronucleus.According to our knowledge this is the first time that the anticlastogenic effect of vitamin A and E post and past irradiation in in vivo has been studied at the same time using micronucleus assay