new approach for MRSA blind chemotherapy

ABSTRACT

In a trial to update the blind chemotherapy for methicillin resistant staphylococcus aureus [MRSA], the resistance profiles of six different MRSA isolates, from diabetic foot ulcers, were evaluated using 39 different antibiotics representing 6 groups. MIC values for individual antibiotics and in combination with each of garlic oil [GO] from Allium sativum, Nigella sativa oil [NSO], or olive [Olea europea] leaf extract [OLE] were determined and statistically analyzed. Extracellular protein profiles of each MRSA isolates under the action of each antibiotic alone or in combination with each herbal product were determined and compared via SDSPAGE. Also, the proteolytic activity [as a parameter of bacterial virulence] was visualized using the non-denatured gel electrophoresis. None of the six MRSA isolates found to have the same antibiotic resistance fingerprint. The same was observed for both extracellular proteins profiles and the proteolytic activities. Average MICs for different antibiotic groups was cephalosporins > penicillin > amino-glycosides > Miscellaneous> macrolides > Ouinolones. Addition of the three herbal extracts to different antibiotics was obviously synergistic. Order of synergistic capacity with different antibiotics was generally NSO > GO > OLE. Among Aminoglycosides, gentamicin and tobramycin were the best; for cephalosporins, cefipime and cefpirome; for penicillins, penicillin G and ampicillin; for macrolides, roxithromycin and azithromycin; for quinolones, levofloxacin and ciprofloxacin; imipenim and spectinmycin from the miscellaneous group