Detection and genotyping of hepatitis D virus from HBsAg positive patients in Iran using RT-PCR

ABSTRACT

Hepatitis Delta virus [HDV] is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus [HBV]. Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequences corresponding to the Hepatitis delta antigen [HDAg] C-termini were used for genotyping. The results showed that 63.6% [23 of 36] of [HDAb] positive serum samples [as determined by ELISA] were also positive for HDV-RNA. Sequencing and phylogenic analysis of three Iranian HDV isolates revealed the most homology [93%] with an Italian isolate indicating a close relationship and probably a common origin for these isolates