Serum level of interleukin-10 with its gene polymorphism can be predictors of response to treatment in Egyptian patients with chronic hepatitis C virus

ABSTRACT

The aim of this study was to demonstrate the role of interleukin-10 [IL-10] gene polymorphism and its serum level in predicting response to treatment in patients with chronic hepatitis C virus. This study was carried out on 35 Egyptian patients with chronic HCV [Hepatitis C Virus] and 15 age- and sex-matched healthy subjects as control. They were divided as follows Group I 35 chronic HCV patients. They were subdivided according to their response to combination therapy of pegylated interferon alpha 2b and ribavirin into Group I [a] 21 responder patients. Group I [b] 14 non responder patients. Group II 15 healthy subjects as a control group. IL-10 serum level was assessed by ELISA [Enzyme Linked Immunosorbent Assay] before, during and after treatment. IL-10 gene polymorphism and genotype were analyzed using polymerase chain reaction-restriction fragment length polymorphism [PCR-RFLP]. A significant higher level of serum IL-10 was detected in HCV patients compared to the control group. A significant reduction was detected during treatment and a persistent decrease was found in patients with SVR. Low serum level of IL-10 pretreatment was associated with high treatment response. High pretreatment of the serum level of IL-10 was associated with the severity of chronic necroinflammation and non response to treatment. A positive correlation was found between IL-10 and serum ALT. The frequency of IL-10 592 genotype polymorphism was higher in HCV patients compared to control. A significant higher frequency of the IL-10 592 C/C polymorphism was found in the responder group compared to non responder. No correlation was observed between IL-10 polymorphism and liver histopathology. Serum IL-10 level pretreatment is useful for predicting treatment response in HCV patients. IL-10 may be a useful marker to assess necroinflammation and to monitor the evolution of liver damage. IL-10 gene polymorphism has no relation to liver histopathology. IL-10 592 C/C genotype was more frequent in responder patients