Study of DNA flowcytometry, serum CEA, CA 19-9 in correlation with histopathological staging and grading of colorectal carcinoma

ABSTRACT

Colorectal carcinoma CRC is estimated to have a high incidence among all cancer diagnosis in both sexes. The present work aimed at studying the growth parameters of CRC by measurement of DNA content by flowcytometry and to correlate these parameters with those of adjacent mucosa and those of normal control. Also to correlate these parameters with staging, grading of the tumour and also with serum level of CEA and CA 19-9. Flowcytometric analysis yielded aneuploidy in 65% of cases. Aneuploid tumours were associated with advanced stage and poor differentiation, but the relation between ploidy and both staging and grading of the tumours was insignificant. DI correlated significantly with staging and grading of tumours. S.PF and PI correlated significantly with staging and grading of the tumour and with preoperative value of tumour markers