Evaluation of antinociceptive action of various fractions of methanolic extract of Terminalia bellirica roxb on diabetic neuropathic rats

ABSTRACT

The study aimed to investigating the antinociceptive action of various fractions of methanolic extract of Terminalia Belliriccz roxb on Diabetic neuropathic rats. Diabetes was induced in over night fasted male adult Sprague Dawely rats by a single dose of intra peritoneal injection of streptozotocin 50mg/kg. Animals were divided into 8 groups, and treatment was started on 4[th] week with n-Butanol fraction [200 mg/ kg, p.o.], Ethyl acetate fraction [200 mg/ kg, p.o.], Water fraction [200 mg/ kg, p.o.], Fluoxetine [14.5 mg/kg, p.o.], Imipramine [10.5 mg/kg, p.o.] and Gallic acid[50 mg/kg, p.o.].Pain threshold has been evaluated in each week with analgesic models like Eddys hot plate method, Tail immersion method and Formaline test. Animals treated with Fluoxetine, Imipramine, Gallic Acid, n-butanol, Ethyl acetate and Water fractions has given significant result when compared with Diabetic control in each analgesic models. Among the fractions the result varies with the amount of Gallic acid present in it. Antinociceptive properties of various fractions of methanolic extract of Terminalia bellirica is due to the presence of Gallic acid and effect is through the restoration of brain monoamines, like nor epinephrine, 5-hydroxy tyrptamine [5-HT] and dopamine level