Antagonism of levcromakalim by glibenclamide [glyburide] in the uterus of rat in vitro

ABSTRACT

This study was conducted in the department of Pharmacology, Shaikh Zayed, Post Graduate Medical Institute, Lahore. In the present study the Antagonistic [Inhibitory] effect of Glibenclamide [Glyburide] an oral anti-hyperglycemic agent, on relaxant effect of Levcromakalim [a Potassium Channel Opener] on the non Pregnant isolated rat uterus was studied. Twenty Five albino rats [Sprague Dawley Strain] were sacrificed. The rats were injected with diethyl stilbestrol [estrogen] intra muscularly, 24 hours before the experiments to facilitate the contractile effect of oxytocin on isolated rat uterus. The dose of oxytocin [Mean +/- SEM] 4.360 +/- 0.644ng was required to produce sub-maximial contraction of the uterus. The dose of Levcromakalim [Mean +/- SEM] 3.041 +/- 0.364ug was required to decrease / inhibit 50% contraction of uterus induced by oxytocin. In the presence of Glibenclamide, the dose of Levcromakalim was required [Mean +/- SEM] 7.41 +/- 0.834ug [<0.05] to reduce 50% contraction of the uterus produced by oxytocin