Improved survival and hematopoietic differentiation of murine embryonic stem cells on electrospun polycaprolactone nanofiber

ABSTRACT

Objective: Three-dimensional [3D] biomimetic nanofiber scaffolds have widespread applications in biomedical tissue engineering. They provide a suitable environment for cellular adhesion, survival, proliferation and differentiation, guide new tissue formation and development, and are one of the outstanding goals of tissue engineering. Electrospinning has recently emerged as a leading technique for producing biomimetic scaffolds with micro to nanoscale topography and a high porosity similar to the natural extracellular matrix [ECM]. These scaffolds are comprised of synthetic and natural polymers for tissue engineering applications. Several kinds of cells such as human embryonic stem cells [hESCs] and mouse ESCs [mESCs] have been cultured and differentiated on nanofiber scaffolds. mESCs can be induced to differentiate into a particular cell lineage when cultured as embryoid bodies [EBs] on nano-sized scaffolds