Anti-inflammatory effect of methanolic extract of Conyzacanadensis in lipopolysaccharide [LPS]-stimulated RAW264.7 murine macrophage cells

ABSTRACT

The aim of this study was to investigate the potential anti-inflammatory effect of Conyzacanadeusis methanol extract [CME] using a cell model of RAW264.7 murine macrophage cell stimulated with lipopolysaccharide [LPS][1 microg/ml]. Co-treatment with different concentrations [10, 50 and 100 microg/ml] of CME was concentration-dependently reduced the LPS-induced generation of prostaglandin E2 [PGE2], nitric oxide [NO] tumor necrosis factor-alpha [TNF-alpha], interleukin [IL]-1beta and IL-6. In addition, CME also reduced the mRNA expressions of cyclooxygenase-2 [COX-2], inducible nitric oxide [iNOS], TNF-alpha, IL-1beta and IL-6 in LPS-stimulated RAW264.7 cells. These results suggested that CME showed an anti-inflammatory activity through reduced the mRNA expression of COX-2, iNOS, TNF-alpha, IL-1beta and IL-6 and also decreased the productions of PGE2, NO, TNF-alpha, IL-1beta and IL-6in LPS-stimulated RAW264.7 cells