Assessment of three blood genomic-DNA preparation methods for malaria molecular diagnosis

ABSTRACT

Species-specific PCR techniques are highly sensitive and reliable alternatives to clSpecies-specific PCR techniques are highly sensitive and reliable alternatives to classical methods for malaria diagnosis and speciation, especially in endemic regions under advanced control or elimination programs where asymptomatic and low-density infections are increasingly reported. Nevertheless, the performance of these techniques is directly affected by the quality of isolated DNA templates. A Plasmodium falciparum/vivax-specific diagnostic Nested- PCR [Pf/Pv N-PCR] was used to assess three DNA preparation methods, Qiagen[registered sign] Mini- Chromatographic kit [QIAmp[registered sign]] and Jena-Biosciences[registered sign]DNA isolation kit [JB[registered sign]] for genomic DNA extraction from EDTA-preserved whole blood samples, and Whatman-FTA[registered sign] purification reagent [FTA[registered sign]] for DNA preparation from dry blood spots [DBS] collected onto FTA[registered sign]- cards