Acetyl COA carboxylase in mammary gland and liver of lactating rabbit: effect of opioids

ABSTRACT

The activity of Acetyl COA Carboxylase [ACC] in the mammary gland and liver was investigated in lactating rabbits when administered with morphine and other opioid peptides. The enzyme activities in the tissue of the treated animals are expressed as in vivo studies. In the in vitro experiments, the tissues used were from the untreated groups where the enzyme assay mixture contained added opioids. The in vivo studies show that the activity of enzyme had slight increase in both mammary gland and liver with the administration of opioids. As far asopioid peptides are concerned, there appears to be no change in ACC level both in mammary and liver tissue. Addition of the increasing concerntration of morphine in vitro assay system could cause a significant decrease in mammary and liver tissu. Increasing concentration of methionineenkephalin [Met-Enk], Leucine Enkephalin [Lea -Enk] and Bcasomorphine [B-CM] also caused a significant decrease in both mammary and liver ACC level. Opioid and opioid peptides induced decrease in the enzyme activity was counteracted by naloxone in the in vitro assay system