[Analysis of humoral immunity in broilers challenged with Eimeria prior to and following anticoccidial vaccination, by means of ELISA]
Journal of Veterinary Research. 2015; 70 (3): 279-284
en Fa
| IMEMR
| ID: emr-181008
Biblioteca responsable:
EMRO
Background: Despite the use of prophylactic chemotherapy and vaccination, coccidiosis is still one of the most devastating diseases in poultry industry. Understanding the immune mechanism helps researchers to prevent this parasitic infection more effectively
Objectives: The purpose of this study was to evaluate the antibody response in chickens, induced by a live attenuated vaccine [Livacox Q], before and after challenge, by means of ELISA
Methods: One hundred and twenty one-day-old male Ross 308 broiler chicks were randomly divided into 4 groups of 30 birds. In 4th day of age, half the birds were orally vaccinated. The challenged groups received the infective dose at 14th day of age via oral administration. Besides recording weight gain, lesion score and oocyst count in 21[st] day old birds, humoral immunity was assessed by means of ELISA on serum samples taken from 7 and 21day-old birds
Results: Three days post vaccination, the average of optical density [OD] showed significant difference between vaccinated [0.553] and unvaccinated [0.686] birds [p Conclusions:The results indicated that ELISA can be used for evaluating immunity uniformity of a flock after vaccination. Besides inducing antibody responses comparable to challenge with wild oocysts, vaccination with live attenuated coccidiosis vaccines may have inhibitory effects in intestinal lesion scores which are responsible for pathogenesis and economic loss during coccidial infections
Objectives: The purpose of this study was to evaluate the antibody response in chickens, induced by a live attenuated vaccine [Livacox Q], before and after challenge, by means of ELISA
Methods: One hundred and twenty one-day-old male Ross 308 broiler chicks were randomly divided into 4 groups of 30 birds. In 4th day of age, half the birds were orally vaccinated. The challenged groups received the infective dose at 14th day of age via oral administration. Besides recording weight gain, lesion score and oocyst count in 21[st] day old birds, humoral immunity was assessed by means of ELISA on serum samples taken from 7 and 21day-old birds
Results: Three days post vaccination, the average of optical density [OD] showed significant difference between vaccinated [0.553] and unvaccinated [0.686] birds [p Conclusions:The results indicated that ELISA can be used for evaluating immunity uniformity of a flock after vaccination. Besides inducing antibody responses comparable to challenge with wild oocysts, vaccination with live attenuated coccidiosis vaccines may have inhibitory effects in intestinal lesion scores which are responsible for pathogenesis and economic loss during coccidial infections
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Índice:
IMEMR
Idioma:
Fa
Revista:
J. Vet. Res.
Año:
2015