Why cannot a beta-lactamase gene be detected using an efficient molecular diagnostic method?

ABSTRACT

Objective: Fast detection of beta-lactamase [bla] genes can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited bla gene types, these methods have significant limitations, such as their failure to detect almost all clinically available bla genes. We have evaluated a further refinement of our fast and accurate molecular method, developed to overcome these limitations, using clinical isolates