Effects of ginseng-spikenard heart-nourishing capsule on inactivation of C-type Kv1.4 potassium channel

ABSTRACT

This research is to explore the effects of traditional Chinese medicine Ginseng-spikenard heart-nourishing capsule on the inactivation of c-type Kv1.4 channels [Kv1.4[DELTA]N] in Xenopus laevis oocytes with two-electrode voltageclamp technique. Defolliculated oocytes [stage V-VI] were injected with transcribed cRNAs of ferret Kv1.4[DELTA]N channels. During recording, oocytes were continuously perfused with ND96 solution [control group] and solution prepared from Ginseng-spikenard heart-nourishing capsule [experimental group]. Results found that, at the command potential of +50 mV, the current of experimental group was reduced to 48.33+/-4.0% of that in control group. The inactivation time constants in control and experimental groups were 2962.56+/-175.35 ms and 304.13+/-36.22ms, respectively [P<0.05, n= 7]. The recovery time of fKv1.4[DELTA]N channel after inactivation in control group and experimental groups was 987+/-68.39 ms and 1734.15+/-98.45 ms, respectively [P<0.05, n=5]. Ginseng-spikenard heart-nourishing capsule can inhibit the Kv1.4[DELTA]N channel, which may be one of the mechanisms of underlying antiarrhythmia