Evaluation of recombinant human growth hormone secretion in E. coli using the L-asparaginase II signal peptide

ABSTRACT

Background: In the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. Signal peptides play a critical role in prosperous secretion of recombinant proteins. Accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-error experiments. The aim of this study was to evaluate the effect of L-asparaginase II signal peptide on the recombinant human Growth Hormone [hGH] protein secretion in the Escherichia coli [E. coli] host