[Comparison of two different immature mouse ovarian vitrification methods using trypan blue staining]

ABSTRACT

Objective: Different cryoprotectants are used for cryopreservation of ovarian tissue in patients at risk of infertility. Ethylene glycol [EG], dimethyl sulfoxide [DMSO] and propanediol [PROH] have been chosen as the basic permeable cryoprotectants due to their decreased glass-formation characteristics compared to other cryoprotectants. In the present study, the effects of two different vitrification methods on whole mouse ovarian tissue by the use of a novel staining method [trypan blue] has been evaluated