Coenzyme Q[10] Improves Developmental Competence of Mice Pre-antral Follicle Derived From Vitrified Ovary

ABSTRACT

The aim of this study was to investigate the in vitro development of isolated pre-antral follicles derived from vitrified ovaries in the presence of coenzyme Q[10] [CoQ[10]]. Mice pre-antral follicles derived from fresh and vitrified-warmed ovarian tissues were cultured individually in alpha-MEM medium supplemented with or without CoQ[10], followed by adding human Chorionic Gonadotropin [hCG] to induce ovulation. The follicle development parameters and ovulated oocyte maturation were assessed. The developmental parameters of pre-antral follicles and oocyte maturation rates were significantly higher in CoQ[10] pretreatment groups of both vitrified and fresh samples compared to the respective CoQ[10] free groups. CoQ[10] improves the in vitro development of pre-antral follicles derived from fresh and vitrified -warmed ovaries