Development of flow cytometry-fluorescent in situ hybridization [Flow-FISH] method for detection of PML/RARa chromosomal translocation in acute promyelocytic leukemia cell line

ABSTRACT

Background: acute Promyelocytic Leukemia [APL] is a subclass of acute myeloid leukemia. The chromosomal aberration in 95% of APL cases is t[15; 17] [q22; q21], which prevents cell differentiation. Characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. The goal of this study was to develop a new and powerful Flow- FISH technique to detect the long isoform [L] of PML-RARa fusion transcript in NB4 cell line