In vivo anti-tumor effect of DC-CIK cells on human lymphoma cell line Raji
Pakistan Journal of Pharmaceutical Sciences. 2017; 30 (3 [Special]): 1075-1079
en Inglés
| IMEMR
| ID: emr-189313
ABSTRACT
To research on the effect of DC-CIK cells on human lymphoma cell line Raji the immunophenotype of DCCIK cells was analyzed using flow cytometry, and its proliferation inhibition effect was detected using MTT assay. 24 nude mice [4-6 weeks old] were employed and inoculated Raji cells on right axillaries for constructing human Burkitt lymphoma model. MTT results showed that DC-CIK cells had a significant inhibitory effect on Raji cells with obvious dose- and time- dependent effect. Western Blot results confirmed that DC-CIK cells could significantly down regulate the expression of BCL-2 [P<0.05]. DC-CIK cells possesses significant anti-tumor effect on human Burkitt lymphoma bearing nude mice, and down regulation of Raji induced BCL-2 cell apoptosis may be one of the inhibitory mechanisms of DC-CIK cells
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Células Dendríticas
/
Inmunofenotipificación
/
Linfoma de Burkitt
/
Apoptosis
/
Línea Celular Tumoral
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Células Asesinas Inducidas por Citocinas
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Ratones Desnudos
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Antineoplásicos
Límite:
Animales
Idioma:
Inglés
Revista:
Pak. J. Pharm. Sci.
Año:
2017
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