Expression optimization of anti-CD22 scFv-Apoptin fusion protein using experimental design methodology
IBJ-Iranian Biomedical Journal. 2018; 22 (1): 66-69
en Inglés
| IMEMR
| ID: emr-190549
ABSTRACT
Background:
Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein
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Índice:
IMEMR (Mediterraneo Oriental)
Idioma:
Inglés
Revista:
Iran. Biomed. J.
Año:
2018
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