development of a chemiluminescence-immuno-assay for the measurement of progesterone in saliva

ABSTRACT

An assay for the determination of the minute concentrations of progesterone in saliva using the novel and non-isotopic technique of chemiluminescence immunoassay is described. The assay has been validated and shows a high degree of specificity, sensitivity, precision and hence accuracy. The serial determination of progesterone concentration in the saliva of 14 human volunteers with regular menstrual cycles showed a well defined pattern similar to its pattern in plasma