Analysis of 5HT3Ra gene expression by real time PCR in systemic lupus erythematosus [SLE] patients

ABSTRACT

Systemic lupus erythemathosus is an autoimmune disease that affected many various types of tissues in 10% of world population and over 30 genes has associated with it. Nouroimmunoendocrynology concepts have shown that immune system could be affected by neuron system and vice versa, 5-hydroxytryptamine receptor a [5HT3Ra] was studied as a main receptor in these relations. In this study, peripheral blood sample were collected from [SLE] patient and normal individuals. The total cellular RNAs were extracted and the cDNAs were synthesized. This process was followed by real-time PCR using specific primers for 5HT3Ra gene and beta-actin gene as internal control. Eventually PCR products have been sequenced. Results of this study suggested that this special receptor expressed in polymorpho-nuclear cells. We found over expression of 5HT3Ra in patients in comparison with healthy individuals group. Interestingly, some nucleotide changes have been found in 5HT3Ra gene in patients but not found sequential nucleotide changes in healthy individuals group. This study supposed that over expression of 5HT3Ra gene in SLE patients lead to over activation of immune cells that derived from over stimulation of them from serotonin blood serum that finally lead to autoimmune reactions that terminated in SLE