Polymerase chain reaction versus other diagnostic techniques for detection of helicobacter pylori in gastric biopsy specimens
EJMM-Egyptian Journal of Medical Microbiology [The]. 2011; 20 (3): 57-66
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| ID: emr-195410
Biblioteca responsable:
EMRO
Introduction: Helicobacter Pylori is a common bacteria found in the stomach that can cause ulcers and more rarely gastric cancer. There are multiple diagnostic tests that are currently available to detect this microorganism possibly making it difficult for providers to choose the most accurate or inexpensive test. It is important to diagnosis H. pylori in symptomatic patients due to the potential of eliciting a series of pathologic consequences in the extra-gastric regions. These consequences have been studied at length and can include cardiovascular and autoimmune disorders as well as certain digestive disorders such as liver disease
The Aim of this Study: the aim of this study was to evaluate molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on analysis of urease A [ureA] gene by real-time polymerase chain reaction [PCR], with the other diagnostic methods including culture, histopathology and rapid urease test [RUT] as invasive tests and serology as a non-invasive test in Sohag University Hospitals
Material and Methods: patients complaining of symptoms of upper gastrointestinal disturbance, at the Sohag University hospital from gastroenterology clinic that were already scheduled for upper endoscopy were eligible for enrollment in the study. At the time of endoscopy, 4 biopsies were collected and used for rapid urease, histology, and culture, PCR respectively, urea breath test performed, Stool and serum samples were tested for the presence of H pylori by using commercially available enzyme-inked immunosorbent assay-based technology [ELISA]
Results: positive result for both histopathology and RUT was used as a gold standard for diagnosis against which, sensitivity and specificity of all performed diagnostic tests were calculated. Sensitivity and specificity of PCR were 96.4% and 95.5% respectively with performance index [accuracy] of 96 % which is comparable to that of histopathology [98%] and higher than those of culture [86%], RUT [88%] and serology [70%]
Conclusion: the PCR based molecular method were apparently high sensitive direct method for detection of H. pylori infection from gastric biopsy specimens when compared to standard histologic examination and rapid urease test
The Aim of this Study: the aim of this study was to evaluate molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on analysis of urease A [ureA] gene by real-time polymerase chain reaction [PCR], with the other diagnostic methods including culture, histopathology and rapid urease test [RUT] as invasive tests and serology as a non-invasive test in Sohag University Hospitals
Material and Methods: patients complaining of symptoms of upper gastrointestinal disturbance, at the Sohag University hospital from gastroenterology clinic that were already scheduled for upper endoscopy were eligible for enrollment in the study. At the time of endoscopy, 4 biopsies were collected and used for rapid urease, histology, and culture, PCR respectively, urea breath test performed, Stool and serum samples were tested for the presence of H pylori by using commercially available enzyme-inked immunosorbent assay-based technology [ELISA]
Results: positive result for both histopathology and RUT was used as a gold standard for diagnosis against which, sensitivity and specificity of all performed diagnostic tests were calculated. Sensitivity and specificity of PCR were 96.4% and 95.5% respectively with performance index [accuracy] of 96 % which is comparable to that of histopathology [98%] and higher than those of culture [86%], RUT [88%] and serology [70%]
Conclusion: the PCR based molecular method were apparently high sensitive direct method for detection of H. pylori infection from gastric biopsy specimens when compared to standard histologic examination and rapid urease test
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Índice:
IMEMR
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Egypt. J. Med. Microbiol.
Año:
2011