[Serum levels of antioxidant enzymes, MDA and its relation to T-786C nitric oxide synthase gene polymorphism in patients with Coronary Artery Diseases [CAD]]

Background and Objectives: Various mutations on endothelial Nitric Oxide synthase [eNOs] gene can cause reduced production of NO and may accelerate the process of atherosclerosis. The study was designed to investigate the frequency of T-786C gene polymorphism in a group of patients suffering from CAD in North West of Iran, The association of the polymorphism with other risk factors including: Antioxidant activity indices, Lipid Peroxidation and MDA were also determined

---

Material and Methods: One hundred twenty subjects including 60 patients with angiographically diagnosed CAD and 60 age and sex matched normal control were studied. Exclusion criteria were; diabetes melitus smoking, preexisting malignancies and liver disease. The activities of SOD, GPX and TAC enzymes in the blood samples were determined in both groups using Randox Kits in an automated chemical analyzer [Abbott, model Alcyon 300,USA]. The concentration of MDA in the serum samples were assayed by spectrophotometerically using Thiobarbitoric acid reaction. The genotype studies were also carried out concomitantly using allele specific PCR

---

Results: Erythrocyte SOD, GPX activities and blood TAC level were reduced significantly in patients with CAD [P<0.05 in all cases]. The levels of serum MDA in CAD patients were significantly higher than controls [P<0.05]. The significantly higher frequency of eNOs -786C genotype was detected in CAD patients that indicate the significant association of C allels with CAD

---

Conclusion: The association between oxidative stress parameters and decrease Antioxidant activity may suggest their involvement in the pathogenesis of atherosclerosis. Increased frequency of T-786C polymorphism might be another risk factor in the progression of coronary artery disease