Tissue culture of ajowan-e-khurasani [hymoscyamus niger L]

ABSTRACT

Callus cultures were initiated from redical, leaf, petiole, stem and root explants of H. niger. MS medium with kinetin and 2,4-D at a concentration of 0.1 mg/1 was found suitable for good amount of callus formation. Callus was propagated in undifferentiated form, for more than a year with the addition of CH, CM and SDC to the culture medium. The presence of alkaloids in cultures was observed dependent on the colour and origin of the callus