Effect of the organophosphate [curacron] on activity of acetylcholinesterase in different parts of rat brain [in vivo studies]

ABSTRACT

Curacron profenofos is 0-[4-Bromo-2 chlorophenyl]-O-ethyi-S-propyl phosphorothioate. Its chemical formula C11H15BrCIO3PS. It is a broad-spectrum organophosphorous insecticide for control of pests in cotton growing areas. Inhibition of acetyi and pseudocholinesterase by this compound results in signs and symptoms of toxicity signs and symptoms [cholinergic syndrome] bradycardia, respiratory-circulatory arrest, vomiting and diam'iea in a variety of animals including man. In this work experiments were carried out in vivo to study its effect on the activity of AChE enzyme obtained from whole and five different parts of rat brain, namely basal ganglia, frontal cortex, medulla oblongata, pons, and cerebellum. This work was carried out by 2 experiments Exp. A Depending on the dose of intraperitoneal infusion of curacon. Exp. B Depending on the time course post infusion of a constant dose of curacron 100 mg/kg BW [dose causing 50% inhibition] in vivo. The results showed that the inhibition occurred in all brain parts, but it was highly marked in the enzyme obtained from the cerebellum [the part responsible for balance and coordination of movements]. The results also indicated that the inhibition of AChE produced by i.p. injection with curacon was dose and time dependent