Cellulases production from wastes under different conditions

ABSTRACT

The optimal conditions were tested to achieve the highest enzymes production by fermentation. Beet pulp and olive cake, some of the predominant waste materials in Egypt, were checked as base media with respect to cellulases production. The maximum enzyme activities of cellulases produced by Trichoderma reesei and Sclerotium cepivorum were estimated to verify the optimal conditions. The optimal conditions were, 12 day of incubation period, 30 degree C as optimum temperature, 5.0 as pH value and 4% as inoculum size, for T. reesei. The corresponding values for S. cepivorum were 16 days, 21 degree C, 4.5 and 2%. The optimum shaking rate for T. reesei was 200 rpm