T-2 toxin control and toxigenicity on adrenal gland

ABSTRACT

In the present study, extraction of T-2 toxin is proved to be maximum using 0.5% NaCl in a liquid medium containing cellulose [Carbon source], shaking growth [140 r.p.m.] for 14 days at 25°C was done based on TLC and UV spectrophotometric analyses. Notice extraction is slightly decreased with increasing NaC1 until 2.5%, followed by sharply depressed, where T-2 toxin could not be detected In the extract at 3.5% NaCl. Two treated groups of male albino rats [each, 10 rats] injected by T-2 toxin used in this study. The blood samples from the heart of the first group [one dose, 0.1 mg/kg body weight for one month] showed a decrease of plasma level of cortisol, cholesterol and triglyceride with increasing of TBARS than previous control group. Also, the second group [two doses, each 0.1 mg/kg body weight, for two months] had the same remits of the first treated group. Histological study of adrenal cortex shows significant alteration in the cells of both zona glomerulosa and zona fasciculata after one month of T-2 toxin administration. The latter layer shows hypertrophy of the cells with vacuolation of the cytoplasm and pyknosis of their nuclei which become small and deeply stained. These changes become less. apparent after 2 months, where the gland become more or less similar to control. The histology of the gland explain what was found In biochemical assays. These results were discussed In view of the available literature