neutrophil phagocytic activity and the effect of liquid preservation

ABSTRACT

The phagocytic activity of polymorphonulcer leukocytes was studied after Collection in different anticoagulants. The effect of storage in different anticoagulants and storage media was studied after 24,48 and 72 hr of collection Fivety one blood samples were taken from Mansoura University Hospital blood hank donors by phlebotomy into plastic bags using citrate phosphate dextrose [CPD], citFate Phosphate dextFose adenine[CPD-A] and heparin Then, stored under standard blood bank conditions in different storage media i.e. whole blood leukocyte-rich plasma and synthetic media [RPMI 1940 and Medium 199]. Granulocytes were separated and their phagocytic activity was assessed immediately after collection, then after 24, 48, and 72 hr of Storage. A comparative study was done between neutrophil phagocytic activity after storage in different anticoagulants and also between four different preservative media. The comparative study between anticoagulants showed that CPD and CPDA were superior to heparin As regards preservative media leukocyte rich plasma was the best medium