Anaerobes in female genital tract potential infection, respiratory infection and post-operative wound infection: a concise study

ABSTRACT

Sixty cases were included in this study selected as 30 with female genital tract potential infection, preterm premature repture of membranes, 20 with respiratory infection and 10 with post-operative wound infection. Cases were subjected to different techniques for primary isolation of anaerobes. Anaerobic isolates were further identified, characterized and submitted to antibiotic susceptibility to 5 antibiotics using the disc diffusion method. Twenty nine cases [43.3%] were positive for an aerobes 12/30 in female genital tract potential infection group [40%], 12/20 in respiratory infection group [60%] and 5/10 in postoperative wound infection group [50%]. Bacteroides were the most frequently isolated anaerobes, secondly isolated was fusobacteria followed by viellonella, peptostreptococci and lactobailli. The highest results were yielded by direct plating on non-selective media and by enrichment in liquid media with subculture on selective media. [with cumulation of positive results on different selective media]. Metronidazole and clindamycin were the most active antimicrobial agents against anaerobes. The present study adds to the concept of using more than one technique for better yield of isolation and the utmost importance of incorporation of metronidazole and clindamycin in any antibiotic consideration