Some biophysical parameters for detection of heterozygotes B-thalassemia

ABSTRACT

Heparinized blood was taken from cases of homozygotes Beta and Beta [+] thalassemic children together with their parents as heterozygotes. In addition, samples were taken from iron deficiency anemia, glucose-6- phosphate dehydrogenase deficiency [G-6-pD], sickel cell anemia and normal control samples. Hemoglobin absorption spectra, base and acid buffer capacities, electrophoretical pattern, thermodynamic parameters, conductivity and viscosity of Hb were investigated. The obtained data revealed a four times in base buffer capacity regarding homozygotes compared with control and two time regarding heterozygotes. Hyperchromicity of globin band and half width of the area under Soret band showed a pronounced increase in homozygotes and heterozygotes as well as a drop in A 578/A 542 ratio concomitant with the appearance of a new band at 630 nm. In addition, in homozygotes and heterozygotes a turning point thereshold and decrease in the slope of linear relationship between natural logarithm of spin state constant versus 1/T was detected respectively. The obtained data revealed also that conductivity was 0.7 X 10[-3] [ohm[-1] cm[-1]] and 0.3 X 10[-3] [ohm[-1] .cm[-1]] of control values regarding homozygotes and heterozygotes respectively. Thus, it is easy to detect heterozygotes according ot these biophysical parameters