Isolation, purification and ultrastructure of bacteriophages from natural mosquito breeding places in Egypt

ABSTRACT

Two bacteriophages were isolated from field collected samples representing two different mosquito breeding places. The phage AB-1 [isolated from Abheit Village, Fayoum Governorate "seepage water"] and the phage GA-2 [isolated from El-Gabal El-Asfer, Qalyoubia Governorate "sewage drain water"] were purified. Both bacteriophages were ultrastructurally described with respect to their morphology, dimensions, phases of bacterial attack and lysogeny. No major differences were observed between both isolated phages in relation to specificity, however, they were isolated from two different types of breeding places and two different geographic areas as well. This study may assume a wide host range of the isolated phages and reflect how bacterial insecticides used for mosquito larval control could be inhibited by such bacteriophage