effect of sepsis on the histochemical activity of some hepatic enzymes

ABSTRACT

The effect of sepsis, induced by caecal ligation and puncture, on the histochemical activity of some hepatic enzymes concerned with carbohydrate metabolism was studied in male rats. This activity was assessed by the staining intensity and zonal distribution of the reaction product in the livers of septic rats, the activity of glucokinase was decreased while that of phosphorylase was increased, In contrast the activity of hexokinase was not affected. These findings provided morphological basis for some of the known biochemical results of other researchers. The possible mechanisms and effects of these findings are discussed in morphobiochemical terms. A new histochemical method has been devised in this study for the demonstration of Liver glucokinase and hexokinase separately. Essentially this method depends on the use of an appropriate concentration of a substrate and a modifier