Galactosamine [GaIN] inhibits luminol-dependent chemiluminescence of human polymorphonuclear leukocytes

ABSTRACT

The aim of the present study was to examine the effect of D- galactosamine HCl [GaIN] on the oxidative respiratory burst of isolated human polymorphonuclear leukocyte [PMNs]. GaIN added in vitro to PMNs caused a marked inhibitory effect on the luminol- dependent chemiluminescene [CL] induced by phorbol myristate acetate [PMA] on PMNs. The inhibitory effect produced by GaIN was both dose and time dependent when PMA was used to stimulate the oxidative burst of PMNs. The effect of GaIN on the isolated PMNs was partially irreversible, following washing of the GaIN-treated PMNs with phosphate buffered saline [PBS]. PMNs viability was not significantly altered by incubation with GaIN. Addition of lipopolysaccharide [endotoxin] to isolate PMNs in the presence or absence of GaIN did not alter the oxidative burst of PMNs. Addition of oxygen-free radical scavengers enhanced the inhibitory effect induced by GaIN on PMNs CL. In a cell free medium, GaIN has no inhibitory effect on CL induced by luminol, H2O2 and horse radish peroxidase. As a conclusion, results suggested that in vitro, GaIN has a remarkable inhibitory effect on the release of oxygen products from stimulated PMNs