Estimation of creatine phosphokinase enzyme in the cerebrospinal fluid of hypothermic dogs

ABSTRACT

Creatine phosphokinase enzyme in the cerebrospinal fluid was determined in two groups of animals [Dogs], each comprised 6 dogs. Animals of the first group were anaesthetised and cooled to a body temperature of 29°C, and then rewarmed to 35°C [total time was 5 hours], while animals of the second group [control] were anaesthetised without cooling for a corresponding time. CPK level was significantly increased in the first group in comparison to the control group, the enzyme level remained high for 24 hours after hypothermia, then the values returned to the initial levels after 1 week. This indicate that estimation of CSF CPK can be used in the diagnosis of hypothermtic death