Studies on some factors influencing the production of calcium lactobionate by Pseudomonas mucidolens NRRL B-16

ABSTRACT

The effect of some factors on calcium lactobionate production by P. mucidolens NRRL B-16 using the submerged culture technique was investigated. It was found that optimum yield of calcium lactobionate [65%] was obtained when the culture medium contained [g/L], lactose 100, KH2PO4 0.6, MgSO4.7H2O 0.25, corn steep liquor 5 ml, calcium carbonate 2.5 g/100 ml, 1 ml/100 ml of 20% urea and 3 drops of soybean oil/100 ml as an antiforming agent at an initial pH of 7 after 14 days of incubation at 30C