Studies on the production of maltobionic acid by Pseudomonas mucidolens NRRL B-16 on maltose

ABSTRACT

The effect of different media, pH value [phosphate buffer], different concentrations of corn steep liquor and urea and different concentrations of maltose at different incubation periods on the production of maltobionic acid by P. mucidolens NRRL B-16 using the submerged culture technique was investigated. It was found that optimum yield of maltobionic acid [51.9%] after incubation periods [14 days] could be secured by providing the using of fermentation medium containing [g/L] 100 maltose, 0.6 KH2PO4, 0.25 MgSO4.7H2O, 5 ml of corn steep liquor and three drops of soybean oil were added to each culture as an antifoaming agent. Separately, 1 ml of sterile 20% urea was added to each culture flask at pH 6.98