Enzyme immuno-assay for the detection of urine antibodies in children infected with schistosoma hematobium

ABSTRACT

Enzyme linked Immunosorbant assay [ELISA] and dot- ELISA were performed to detect antibodies to Schisosoma haematobium egg antigens [Sh-SEA] in midday urine samples obtained from parasitological by proven urinary schistosomiases infected children. The assays failed to capture specific antibodies in 25-30 percent of patient's urine samples. Enzyme immunoblot assay [EIB] revealed consistent recognition by all the urine samples from S. haematobium cases to 20-106 KDa antigenic bands of Sh-SEA. The use of urine samples from mansoniasis or normal individuals [n=23] indicated 87 percent diagnostic specificity of this EIB. Thus, immunoreactivity of urine to electrophoresed Sh-SEA antigen is highly sensitive and non-invasive technique that could be asuitable alternative to serum ELISA for diagnosis of haematobia, particularly, for epidemiological studies on children