Effect of pirenzepine [gastrozepin] on the induced duodenal ulcer by aspirin in albino rat

ABSTRACT

Adult albino rats kept on standard diet and water were divided into 2 main groups The first group served as control group, animals were sacrificed after 6 weeks. The second group received a daily oral dose of acetyl salicylic acid [aspirin] 100 mg/kg body weight/day for 6 weeks. The duodenum from all animals were processed to paraffin sections. They were stained with H and F, periodic acid-Schiff's reaction, Alcian blue, Bodian technique and Masson's trichrome stain. Parts of the duodenum were cryostate cut, stained with Gomori's technique for acid phosphate. On aspirin administration, there was discontinuity and loss of the lining epithelium at the top of the villi. Some epithelial cells were undergoing degeneration with decrease in heights. The brush border was interrupted. PAS demonstrated apparent increase in goblet cells, while Alcian blue stained sections appeared paler blue. Acid phosphatase reaction was increased. The lamina propria appeared disorganized with no evidence of fibrosis. Mitotic figures were increased in the base of the crypts. On pirenzepine administration, the duodenum appeared normal resembling control. The villous tips appeared intact. The brush border regained its continuity, goblet cells were apparently decreased reaching control level, while Alcian blue showed increased intensity. The acid phosphatase reaction was reduced