Bacterial multiresistance in clinical practice

ABSTRACT

Over a period of one year, 202 isolates with antibiotic multiresistant pattern [> 5 antibiotics] were selected from different samples received at Ain Shams University Hospitals laboratories. Pure cultures of isolates were identified by cultural characters and biochemical reactions using conventional methods, modified micro well plates and the new technique of agar plates incorportated with chemical substrate. Susceptibility tests were performed by disk diffusion method and by minimum inhibitory concentration using microdilution broth technique. Multiresistant strains were isolated mainly from wound specimens [46.5%] and urine [45.5%]. They were mostly Ps. pyocyaneus [36.6%] followed by Klebsiella [22.7%] and Staph. aureus [17.8%]. Gram negative organisms showed high resistance [> 90%] towards ampicillin / sulbactam [unasyn], cephalexin and nalidixic acid. Also they shared Staph. aureus in their resistance [> 90%] towards amoxycillin and ampicillin. Such results indicate that an alarming increase in antibiotic resistance has taken place among both gr -ve and gr +ve isolates